blot 相关例句
及物动词
1. blot在线翻译
1. He blotted his driving record by having an accident.
他因发生一次事故而在驾驶记录上留下污点。
2. blot的翻译
2. The dense fog blotted out the whole view.
浓雾遮住了整个景色。
3. blot
3. She blotted her copybook when she arrived late for work.
她上班迟到影响了自己的名声。
不及物动词
1. blot什么意思
1. My pen blots easily.
我的钢笔容易漏墨水。
名词
1. Is there any way to remove the ink blot on my dress?
有什么办法去掉我衣服上的墨渍吗？
blot 网络解释
1. blot的反义词
1. 印迹：三、核酸限制性片段长度多态性(RFLP)分析RFLP分析是限制性内切酶、核酸电泳、印迹(blot)技术、探针-杂交技术的综合应用,多用于临床遗传性疾病的基因诊断. 基本原理是遗传性疾病多有基因的缺陷,如基因缺失、基因插入、编码区小范围核苷酸序列改变或点突变等.
2. 玷污,损害风景的东西：9. each punch and chop 每一个击、打 | 10. blot 玷污,损害风景的东西 | 11. tinker 修补,调整
3. blot
3. 污渍, 污点 <单词词性>弄污：a bed of flowers 安乐窝 | blot 污渍, 污点 <单词词性>弄污 | drepaniform 镰形的
4. 龙头妖：Hun-Gurrr 龙头魔 | Blot 龙头妖 | Cutthroad 龙头精
blot 双语例句
1. In this study, FAK content and tyrosine phosphorylation were assessed by Western blot and immunoprecipitation in cultured rat vascular smooth muscle cells.
在体外培养的血管平滑肌细胞上，采用蛋白印迹杂交免疫沉淀的方法，研究尾加压素Ⅱ与粘着斑激酶介导的信号传导之间的关系。
2. Today, many ethnic minority areas in China it still remains a blot. Tibetan areas such as Marriage rush and Cost of Life these two fairly typical customs.
时至今日，我国很多少数民族地区仍遗存着它的印迹，如藏族地区就保留了抢婚和赔命价这两个颇为典型的风俗习惯。
3. /u0064/u0061/u006E/u0063/u0069/u002E/u0039/u0031/u0031/u0063/u0068/u0061/u002E/u0063/u006F/u006D
3. Moreover, ELISA protocol has been established with the two kind of antibodies.1 Preparation and identification of anti-hBLyS polyclonal antibodyJapanese big ear white male rabbits (2~3 kg) were injected with soluble hBLyS emulsified with Freund's adjuvant every two weeks. The rabbits were bleed from the ear artery on the 11~th day since the third time immunization, and the titer of antisera was tested by indirect ELISA. Antisera titer reach to 1:5 × 10~5. Rabbits were killed, and blood was collected. Antisera was prepared and purifid with saturated ammonoium sulfate.
抗hBLyS多克隆抗体的制备与鉴定选取2～3kg的雄性日本大耳白兔，淋巴结注射弗氏佐剂乳化的可溶性hBLyS蛋白，每间隔2周注射一次；第3次免疫后11天，耳动脉采血，间接ELISA检测抗血清的效价为50万倍；颈总动脉放血，制备抗血清，并应用分级饱和硫酸铵盐析法进行纯化，westen-blot分析抗体的特异性。
4. 911查询·英语单词
4. The result of Southern blot and plasmid recovery indicated pcDNA3-EGFP-EBVR and pcDNA3-EGFP-EBNA1-MAR existed as episomal state in COS-7 cells.
由于EBVR的存在，可以在体外获得EGFP较长时间的稳定表达。
5. When compared with control spinal cord samples taken from non-injected animals, ipsilateral expressions of 5-HT〓 receptor mRNA 1 h or 4 h after bee venom injection significantly increased by 80.94% or 37.86%, respectively. There were little changes in 5-HT〓 receptor mRNA level on the contralateral side at these two time points.
本课题联合应用分子生物学方法（半定量RT-PCR、Western Blot）、药理学方法和行为学方法（皮下注射蜜蜂毒诱发炎性痛的行为学观察），研究了脊髓背角内5-HT〓受体在伤害性信息传递过程中的作用。
6. In TUNEL assay it can be observed that cystamine can reduce myocardial cell apoptosis. At the same time Western Blot analysis to prove cystamine can reduce cardiac cell apoptosis and Deth receptor and Mitochondria through theses two pathsway, NF-κB protein expression significantly increased furthermore, it promots cell survival, which can reduce cardiac hypertrophy, apoptosis.
在TUNEL assay中可以观察到胱胺可以减少心肌细胞的凋亡，同时我们也利用Western Blot证明胱胺可以减少心肌细胞的凋亡是透过Deth receptor和Mitochondria这两条路径，而这样子降低凋亡的现象很有可能与NF-kB这讯息传递的分子上升有关，因此胱胺确实具有保护心肌细胞的作用，可改善心肌细胞肥大、凋亡的情形。
7. The detection of rat mesangial cells apoptosis-inhibiting Bcl-2 protein with Western blot: The testing cells washed by PBS were added the loading buffer, and turned to the EP tube to boil, then took the same quaintity protein to nitric acid cellulose film after polyacrylamide gel electrophoresis, next added buffer to block. at last add the anti-body and rabbit anti-Bcl-2 to hatch, flowing with color reaction and taking photo.
免疫印迹法检测调亡抑制基因Bcl-2蛋白的表达：PBS漂洗待检细胞，加入上样缓冲液，转移至EP管中，煮沸；取相同量的蛋白上样进行SDS—聚丙烯酰胺凝胶电泳；将SDS—聚丙烯酰胺凝胶上的蛋白质电转移至硝酸纤维素膜上；以20ml封闭缓冲液，加入检测蛋白的抗体孵育；加入二抗羊抗兔IgG孵育。
8. Methods Cell culture, Fluo-3 AM loading and laser sca ing ceonfocal microscope, flowcytometerand western blotwere employed to detect expre ion and regulation effect on signal tra duction pathway of gap junction gene co exin Cx43 in HeLa cells by retiontic acid, Results After treated with ATRA, the intercellular second me enger di ociated calcium ([Ca2]i)waw much higher in HeLa cells(58.16 μmol/L)than untreated cell 35.73% in RA-treated cells than untreated cell examined by FCM and westem blot.
应用特异性钙指示剂（Fluo-3 AM在激光扫描共焦显微镜下动态观察维甲酸作用后的细胞质内信号转导分子游离钙的分布及强度变化。采用充式细胞仪、结合蛋白印迹技术分析，检测外源信号分子对Cx43的表达以及蛋白酪氨酸磷化状态的影响。结果 HeLa细胞内游离钙经维甲酸作用后明显超载，细胞内游离钙浓度（[Ca2]i）由静息状态下的35.73μmol/L。
9. blot的解释
9. Methods Cell culture, Fluo-3 AM loading and laser sca ing ceonfocal microscope, flowcytometerand western blotwere employed to detect expre ion and regulation effect on signal tra duction pathway of gap junction gene co exin Cx43 in HeLa cells by retiontic acid, Results After treated with ATRA, the intercellular second me enger di ociated calciumwaw much higher in HeLa cells(58.16 μmol/L)than untreated cell 35.73% in RA-treated cells than untreated cell examined by FCM and westem blot.
应用特异性钙指示剂（Fluo-3 AM在激光扫描共焦显微镜下动态观察维甲酸作用后的细胞质内信号转导分子游离钙的分布及强度变化。采用充式细胞仪、结合蛋白印迹技术分析，检测外源信号分子对Cx43的表达以及蛋白酪氨酸磷化状态的影响。
10. 911查询·英语单词
10. After G418 selection for 4 weeks, the cell line with stable expression of the gene was obtained. Then the hBKLF expression level, proliferation ability, colony formation and hemoglobin production were detected by RT-PCR and Western blot, MTT method, methyl cellulose semisolid culture method and benzidine test respectively.
构建hBKLF正义及反义真核表达载体，转染K562细胞，经G418筛选4周，得到稳定细胞株；用RT- PCR、Western blot方法测定转染后K562细胞hBKLF表达水平的变化；以转染空质粒的K562细胞为对照组，在显微镜下直接观察转染正义质粒和反义质粒的两组细胞形态的改变；用MTT法比较增殖速率的变化；用甲基纤维素半固体培养法比较集落形成率的差别；用苯息丁法观察氯高铁血红素诱导K562细胞分化后血红蛋白合成水平的差异。
11. 911查询·英语单词
11. The sense and antisense expression plasmids of hBKLF were constructed, and transfected into K562 cells by lipofectamine. After G418 selection for 4 weeks, the cell line with stable expression of the gene was obtained. Then the hBKLF expression level, proliferation ability, colony formation and hemoglobin production were detected by RT-PCR and Western blot, MTT method, methyl cellulose semisolid culture method and benzidine test respectively.
构建hBKLF正义及反义真核表达载体，转染K562细胞，经G418筛选4周，得到稳定细胞株；用RT- PCR、Western blot测定转染后K562细胞hBKLF表达水平的变化；以转染空质粒的K562细胞为对照组，在显微镜下直接观察转染正义质粒和反义质粒的两组细胞形态的改变；用MTT法比较增殖速率的变化；用甲基纤维素半固体培养法比较集落形成率的差别；用苯息丁法观察氯高铁血红素诱导K562细胞分化后血红蛋白合成水平的差异。
12. Then the hBKLF expression level, proliferation ability, colony formation and hemoglobin production were detected by RT-PCR and Western blot, MTT method, methyl cellulose semisolid culture method and benzidine test respectively.
构建hBKLF正义及反义真核表达载体，转染K562细胞，经G418筛选4周，得到稳定细胞株；用RT- PCR、Western blot方法测定转染后K562细胞hBKLF表达水平的变化；以转染空质粒的K562细胞为对照组，在显微镜下直接观察转染正义质粒和反义质粒的两组细胞形态的改变；用MTT法比较增殖速率的变化；用甲基纤维素半固体培养法比较集落形成率的差别；用苯息丁法观察氯高铁血红素诱导K562细胞分化后血红蛋白合成水平的差异。
13. Meanwhile, those expressing FAE1 genes from Sinapis arvensis, Sinapis alba, and Isatis indigotica produced small quantity of VLCFA which was absent in control yeast cells. The experiment indicated that O. violaceus and C.
Western Blot分析发现野生种的FAE1都在酵母系统中获得了表达，转化诸葛菜和荠菜FAE1的酵母并未合成长链脂肪酸，而转化新疆野芥、新疆白芥和菘蓝的FAE1的酵母都有微量长链脂肪酸的合成。
14. The IgG antibody angainst DNA polymerase of EBV in sera of NPC patients can be testified by expressed DNA polymerase through Western-blot and ELISA.
在NPC病人血清中存在抗EB 秋季病毒 DNA聚合酶的IgG抗体，并证明DNA聚合酶的抗原性主要集中于后2／3片段（A2）上。
15. With the methods of PCR-single strand conformational polymorphism, real time quantitative PCR, immunoprecipitate and Western blot, gene mutation, mRNA content, the expression and phosphorylation of protein and catalysis activity of SHP-1, SHP-2 were detected in radiation carcinogenesis.
在骨髓细胞中，癌变组SHP-2的mRNA含量、蛋白质表达量及酶催化活性均无明显变化。
16. danci.911cha.com
16. At first, we establish the leukemia model induced by γ-ray irradiation in mice and the malignant transformation model in BEAS-2B cell line. With the methods of PCR-single strand conformational polymorphism, real time quantitative PCR, immunoprecipitate and Western blot, gene mutation, mRNA content, the expression and phosphorylation of protein and catalysis activity of SHP-1, SHP-2 were detected in radiation carcinogenesis.
本研究首先建立了γ射线诱发的白血病小鼠动物模型及发生恶性转化的BEAS-2B细胞模型，运用PCR-SSCP、实时荧光定量PCR、免疫沉淀、Western blot等分子生物学及细胞生物学方法，从基因突变，mRNA含量，蛋白质表达量，蛋白质酪氨酸磷酸化水平及酶催化活性等方面多层次地分析了酪氨酸磷酸酶SHP-1、SHP-2在辐射致癌中的作用。
17. The forces used warning blots and tear gases, along with sticks, people were badly injured It reached the point of no return when two persons killed to become the first martyrs of the 6 of April which I always remember, the first one was 20 years old young man and the second was a 9 years old boy strangely today in Gaza also a young boy was killed!!
镇暴警察发射warning blot以及催泪瓦斯驱散民众，有的手持警棍，民众受伤惨重。暴动愈演愈烈，尤其当两位民众意外丧生之后，更是一发不可收拾。我永远不会忘记这两位四月六日丧生的烈士，一位是二十岁的青年，另一位才九岁，「今天真是太奇怪了！
18. Finally, we surveyed the blocking activity of a panel of mAbs against PTA I on the binding of PTA I hg to PTA1L, and found that LeoAl could block this binding completely, which suggested that the epitope recognized by LeoAl on PTAI was possibly the same or adjacent one with which PTA1 could bind to its ligand.
为了确定PTAIL的分子量，我们采用生物素标记技术，对高水平表达 PTAIL的 ECV304细胞进行了生物素标j己，经 Protein －A 刀一＼IgGI预清除后，采用PTAI／Ig融合蛋白进行免疫沉淀，通过 Western.Blot检测发现，PTAIL可能为一分子量大于220kD的蛋白。
19. METHODS: The recombinant eukaryotic expression plasmid, pSecTag2/Hygro B Wnt3a, was transfected into NIH3T3 cells by liposome, then the expressed protein was detected by Western Blot.
应用脂质体转染试剂将重组真核表达载体pSecTag2/Hygro B Wnt3a转染并筛选稳定表达的NIH3T3细胞，Western Blot鉴定重组Wnt 3a蛋白的表达，并对Wnt3a/NIH3T3细胞的融合密度及抗凋亡能力给予检测。
20. The PGE2 in plasm and gastric mucoas were measured by RIA; Immunohistochemical SABC kit, micro-image analysis and the method of Western dot blot were used to measure HSPs.
以放射免疫法检测血浆及胃粘膜匀浆前列腺素E2(PGE2)含量，血清热休克蛋白测定采用Western斑点印迹法，SABC免疫组织化学方法用于观察胃粘膜HSPs并进行图像分析。
blot 词典解释
1. （名誉上的）污点，耻辱
If something is a blot on a person's or thing's reputation, it spoils their reputation.
e.g. ...a blot on the reputation of the architectural profession...
败坏建筑业名声的污点
e.g. This drugs scandal is another blot on the Olympics.
这次的兴奋剂丑闻又给奥林匹克运动会抹了黑。
2. 污渍；污痕；污点
A blot is a drop of liquid that has fallen on to a surface and has dried.
e.g. ...an ink blot.
墨迹
3. （用软纸或布）吸干，擦干
If you blot a surface, you remove liquid from it by pressing a piece of soft paper or cloth onto it.
e.g. Before applying make-up, blot the face with a tissue to remove any excess oils...
上妆前，先用面巾纸把脸上多余的油吸干。
4. 破坏整体美的事物；煞风景的事物
If you describe something such as a building as a blot on the landscape, you mean that you think it is very ugly and spoils an otherwise attractive place.
e.g. The developers insist the 80m village will not leave a blot on the landscape.
开发商们坚称这个耗资8,000万的居住区不会破坏风景。
相关词组：blot out
blot 单语例句
1. Volunteers change the deep fryer's oil after each batch and blot each piece of fried fish dry of extra grease.
2. This is a blot on our society and must be rooted out.
3. Gamma ray bursts would also trigger smog formation that could blot out sunlight and rain down acid.
4. Some netizens are angry, while others even say the sexy photographs are a blot on the image of teachers.
5. For honest operators like Xiang, these cooks from hell are a blot on Guijie's reputation.
6. But there will be one red blot on the report card the green component.
7. But is corruption really the biggest blot on the country's image?
blot 英英释义
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noun
1. an act that brings discredit to the person who does it
e.g. he made a huge blot on his copybook
Synonym: smear smirch spot stain
2. a blemish made by dirt
e.g. he had a smudge on his cheek
blot
verb
1. make a spot or mark onto
e.g. The wine spotted the tablecloth
2. dry (ink) with blotting paper
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